Validation Data Gallery
Filter:
Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-66387 targets GSDMD in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GSDMD fusion protein Ag16827 相同性解析による交差性が予測される生物種 |
| Full Name | gasdermin D |
| Calculated molecular weight | 484 aa, 53 kDa |
| Observed molecular weight | 47-53 kDa |
| GenBank accession number | BC008904 |
| Gene Symbol | GSDMD |
| Gene ID (NCBI) | 79792 |
| RRID | AB_2883574 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P57764 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 GSDMD antibody CL594-66387 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |