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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Gamma Tubulin Monoclonal antibody, PBS Only

Gamma Tubulin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F9H8

Cat no : 66320-1-PBS

Synonyms

TUBG1, TUBG, GCP-1, Gamma-tubulin complex component 1, Gamma-1-tubulin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Gamma Tubulin Monoclonal antibody (66320-1-Ig)

    WB analysis using 66320-1-Ig (same clone as 66320-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Gamma Tubulin Monoclonal antibody (66320-1-Ig)

    WB analysis of HepG2 using 66320-1-Ig (same clone as 66320-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of EC109 cells using Gamma Tubulin Monoclonal antibody (66320-1-Ig)

    WB analysis of EC109 using 66320-1-Ig (same clone as 66320-1-PBS)

    EC109 cells were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using Gamma Tubulin Monoclonal antibody (66320-1-Ig)

    WB analysis of K-562 using 66320-1-Ig (same clone as 66320-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Gamma Tubulin Monoclonal antibody (66320-1-Ig)

    IHC staining of human breast cancer using 66320-1-Ig (same clone as 66320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Gamma Tubulin Monoclonal antibody (66320-1-Ig)

    IHC staining of human breast cancer using 66320-1-Ig (same clone as 66320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Gamma Tubulin Monoclonal antibody (66320-1-Ig)

    IHC staining of human prostate cancer using 66320-1-Ig (same clone as 66320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Gamma Tubulin Monoclonal antibody (66320-1-Ig)

    IHC staining of human prostate cancer using 66320-1-Ig (same clone as 66320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using Gamma Tubulin Monoclonal antibody (66320-1-Ig)

    IF Staining of MDCK using 66320-1-Ig (same clone as 66320-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using Gamma Tubulin Monoclonal antibody (66320-1-Ig)

    IF Staining of MDCK using 66320-1-Ig (same clone as 66320-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Gamma Tubulin Monoclonal antibody (66320-1-Ig)

    IF Staining of A549 using 66320-1-Ig (same clone as 66320-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Gamma Tubulin Monoclonal antibody (66320-1-Ig)

    IF Staining of HeLa using 66320-1-Ig (same clone as 66320-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Gamma Tubulin Monoclonal antibody (66320-1-Ig)

    FC experiment of HeLa using 66320-1-Ig (same clone as 66320-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Gamma Tubulin (66320-1-Ig, Clone:3F9H8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66320-1-PBS targets Gamma Tubulin in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Gamma Tubulin fusion protein Ag23913 相同性解析による交差性が予測される生物種
Full Name tubulin, gamma 1
Calculated molecular weight 51 kDa
Observed molecular weight 48-55 kDa
GenBank accession numberBC000619
Gene symbol Gamma Tubulin
Gene ID (NCBI) 7283
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.