ChromoTek HA-Trap Agarose Kit
The HA-Trap Agarose Kit is a ready-to-use reagent for the IP of HA-tagged proteins. It consists of an anti-HA-tag Nanobody/VHH coupled to agarose beads, along with lysis, wash, and elution buffers to use in the IP process.
Specificity
HA-Tag (sequence YPYDVPDYA)
Applications
IP, Co-IP
Type
Nanobody
Conjugate
Agarose
Cat no : atak
Synonyms
Validation Data Gallery
Product Information
The HA-Trap Agarose Kit is a ready-to-use reagent for the IP of HA-tagged proteins. It consists of an anti-HA-tag Nanobody/VHH coupled to agarose beads, along with lysis, wash, and elution buffers to use in the IP process.
| Description | The HA-Trap Agarose Kit contains HA-Trap agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of HA-tagged proteins. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Works in samples from: mammals, plants, yeast, etc. |
| Applications | IP, Co-IP |
| Specificity/Target | Binds specifically to the HA-tag (sequence YPYDVPDYA) fused to a protein of interest at N-, C- or internal position. Please note that the affinity is highest for a C-terminal fusion. There is no cross-reactivity to other common peptide tags such as the His6-tag, FLAG-tag, Spot-Tag, V5-tag, Strep-tag, or C-tag (other tags not tested). Background binding to host cell proteins from a range of organisms such as human, mouse and dog cell lines or yeast and plants is low. |
| Binding Capacity | 20 ug of recombinant HA-tagged protein (~30 kDa) per 25 uL bead slurry |
| Conjugate | Agarose beads; ~90 um (cross-linked 4% agarose beads) |
| Elution buffer | 2x SDS-sample buffer (Lammli) |
| Wash Buffer Compatibility | 2M NaCl, 5 mM DTT, 0 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0% SDS, 2-3 M Urea |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | 6 nM for C-terminal HA-tags and ca. 180 nM for N-terminal fusions. |
| Compatibility with mass spectrometry | The HA-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_3094560 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze! |
Kit components
| Component | Description |
|---|---|
| HA-Trap Agarose | 20 reactions (500 µl) |
| Lysis buffer | Optimized for cytoplasmic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |