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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FITC Plus-conjugated HIF-1 alpha Polyclonal antibo (FITC-20960)

    IF Staining of HeLa using FITC-20960

    Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using FITC Plus HIF-1 alpha antibody (FITC-20960) at dilution of 1:50.

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  • Featured Product
  • KD/KO Validated

FITC Plus-conjugated HIF-1 alpha Polyclonal antibody

HIF-1 alpha Polyclonal Antibody for IF/ICC
Cat No. FITC-20960

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

HIF1A, HIF 1α, HIF 1 alpha, Class E basic helix-loop-helix protein 78, Basic-helix-loop-helix-PAS protein MOP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  FITC Plus
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inCobalt Chloride treated HeLa cells

Samples need to be treated with hypoxia stimulation.

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-20960 targets HIF-1 alpha in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIF-1 alpha fusion protein Ag15198 相同性解析による交差性が予測される生物種
Full Name hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Calculated molecular weight 826 aa, 93 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC012527
Gene Symbol HIF1A
Gene ID (NCBI) 3091
RRIDAB_3673840
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16665
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .

Protocols

Product Specific Protocols
IF protocol for FITC Plus HIF-1 alpha antibody FITC-20960Download protocol
Standard Protocols
Click here to view our Standard Protocols