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Cardinal Red™-conjugated HIF-1 alpha Polyclonal antibody
HIF-1 alpha Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC (Intra)
Conjugate
Cardinal Red™ Fluorescent Dye
Cat no : CR-20960
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of CR-20960 is HIF-1 alpha Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HIF-1 alpha fusion protein Ag15198 相同性解析による交差性が予測される生物種 |
Full Name | hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) |
Calculated molecular weight | 826 aa, 93 kDa |
Observed molecular weight | 120 kDa |
GenBank accession number | BC012527 |
Gene symbol | HIF1A |
Gene ID (NCBI) | 3091 |
Conjugate | Cardinal Red™ Fluorescent Dye |
Excitation/Emission maxima wavelengths | 592 nm / 611 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .
Protocols
Product Specific Protocols | |
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FC protocol for Cardinal Red™ HIF-1 alpha antibody CR-20960 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |