CoraLite® Plus 647-conjugated IBA1 Recombinant antibody
IBA1 Recombinant Antibody for IF-P, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
IF-P, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
4D5
Cat no : CL647-81728
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | rat brain tissue |
| Positive FC (Intra) detected in | THP-1 cells |
| Positive FC detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
CL647-81728 targets IBA1 in IF-P, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | IBA1 fusion protein Ag1363 相同性解析による交差性が予測される生物種 |
| Full Name | allograft inflammatory factor 1 |
| Calculated molecular weight | 17 kDa |
| Observed molecular weight | 17 kDa |
| GenBank accession number | BC009474 |
| Gene symbol | IBA1 |
| Gene ID (NCBI) | 199 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 IBA1 antibody CL647-81728 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun SOLID: minimizing tissue distortion for brain-wide profiling of diverse architectures |
