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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated IBA1 Recombinant antibody

IBA1 Recombinant Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4D5

Cat no : CL647-81728

Synonyms

AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using CoraLite® Plus 647-conjugated IBA1 Recombinant ant (CL647-81728)

    IF Staining of rat brain using CL647-81728

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 647 IBA1 antibody (CL647-81728, Clone: 4D5 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using CoraLite® Plus 647-conjugated IBA1 Recombinant ant (CL647-81728)

    IF Staining of rat brain using CL647-81728

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 647 IBA1 antibody (CL647-81728, Clone: 4D5 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of THP-1 cells using CoraLite® Plus 647-conjugated IBA1 Recombinant ant (CL647-81728)

    FC experiment of THP-1 using CL647-81728

    1X10^6 THP-1 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human IBA1 (CL647-81728, Clone:4D5) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inrat brain tissue
Positive FC (Intra) detected inTHP-1 cells
Positive FC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-81728 targets IBA1 in IF-P, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IBA1 fusion protein Ag1363 相同性解析による交差性が予測される生物種
Full Name allograft inflammatory factor 1
Calculated molecular weight 17 kDa
Observed molecular weight17 kDa
GenBank accession numberBC009474
Gene symbol IBA1
Gene ID (NCBI) 199
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 IBA1 antibody CL647-81728Download protocol
Standard Protocols
Click here to view our Standard Protocols