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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated ICAM-1/CD54 Monoclonal antibody

ICAM-1/CD54 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F9A8

Cat no : CL594-60299

Synonyms

CD54, ICAM-1, ICAM1, ICAM 1, BB2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CoraLite®594-conjugated ICAM-1 Monoclonal antibody (CL594-60299)

    IF Staining of human lung cancer using CL594-60299

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CL594-60299 (ICAM-1 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CoraLite®594-conjugated ICAM-1 Monoclonal antibody (CL594-60299)

    IF Staining of human lung cancer using CL594-60299

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CL594-60299 (ICAM-1 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CoraLite®594-conjugated ICAM-1/CD54 Monoclonal ant (CL594-60299)

    IF Staining of Raji using CL594-60299

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CoraLite®594 ICAM-1 antibody (CL594-60299, Clone: 2F9A8 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inhuman lung cancer tissue, Raji cells
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60299 targets ICAM-1/CD54 in IF/ICC, IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ICAM-1/CD54 fusion protein Ag8309 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 1
Calculated molecular weight 90 kDa
Observed molecular weight 85-95 kDa
GenBank accession numberBC015969
Gene symbol ICAM-1
Gene ID (NCBI) 3383
RRIDAB_2883457
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Where is ICAM-1 expressed?

Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes1. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.

 What is the function of ICAM-1?

ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-12. In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions3, 4. This protein can be used as an indicator of endothelial activation and of vascular inflammation.

 What is the role of ICAM-1 in disease?

Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material5.

  1.  Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic. Biol. Med. 28, 1379-86 (2000).

  2. Long, E. O. ICAM-1: getting a grip on leukocyte adhesion. J. Immunol. 186, 5021-3 (2011).

  3. Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).

  4. Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr. Opin. Hematol. 22, 53-59 (2015).

  5. Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J. Virol. 77, 6101-7 (2003).


Protocols

Product Specific Protocols
IF protocol for CL594 ICAM-1/CD54 antibody CL594-60299Download protocol
Standard Protocols
Click here to view our Standard Protocols