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  • Featured Product
  • KD/KO Validated

FITC-conjugated ICAM-1/CD54 Monoclonal antibody

ICAM-1/CD54 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P

Conjugate

FITC Fluorescent Dye

CloneNo.

2F9A8

Cat no : FITC-60299

Synonyms

CD54, ICAM-1, ICAM1, ICAM 1, BB2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using FITC-conjugated ICAM-1 Monoclonal antibody (FITC-60299)

    IF Staining of human lung cancer using FITC-60299

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using FITC-60299 (ICAM-1 antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using FITC-conjugated ICAM-1 Monoclonal antibody (FITC-60299)

    IF Staining of human lung cancer using FITC-60299

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using FITC-60299 (ICAM-1 antibody) at dilution of 1:50.

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Tested Applications

Positive IF-P detected inhuman lung cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

FITC-60299 targets ICAM-1/CD54 in IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ICAM-1/CD54 fusion protein Ag8309 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 1
Calculated molecular weight 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC015969
Gene symbol ICAM-1
Gene ID (NCBI) 3383
RRIDAB_2883738
Conjugate FITC Fluorescent Dye
Excitation/Emission maxima wavelengths498 nm / 526 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784). This antibody is FITC conjugated ICAM1 mouse monoclonal antibody (600299-1-Ig) which has passed in WB, IHC and FC detection.

Protocols

Product Specific Protocols
IF protocol for FITC ICAM-1/CD54 antibody FITC-60299Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Appl Bionics Biomech

Exosomes Derived from SW480-Resistant Colon Cancer Cells Are Promote Angiogenesis via BMP-2/Smad5 Signaling Pathway.

Authors - Song Yang
View Article