IFI16 Monoclonal antibody
IFI16 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1C10D7
Cat no : 67790-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HeLa cells, MOLT-4 cells, Ramos cells, Daudi cells |
Positive IHC detected in | human tonsillitis tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67790-1-Ig targets IFI16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | IFI16 fusion protein Ag30314 相同性解析による交差性が予測される生物種 |
Full Name | interferon, gamma-inducible protein 16 |
Calculated molecular weight | 729 aa, 82 kDa |
Observed molecular weight | 85-95 kDa |
GenBank accession number | BC017059 |
Gene symbol | IFI16 |
Gene ID (NCBI) | 3428 |
RRID | AB_2918554 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IFI16 is a member of a family of interferon-inducible nuclear proteins involved in transcriptional regulation functions as a transcriptional repressor. IFI16 may be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. It has been reported that IFI16 participates in the regulation of autophagy, TP53-mediated cell death and innate immune response. IFI16 has different isoforms and 67790-1-Ig antibody detects proteins around 85-95 kDa in SDS-PAGE similar to papers. (PMID: 9718316, 14990579, 11146555, 22046441)
Protocols
Product Specific Protocols | |
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WB protocol for IFI16 antibody 67790-1-Ig | Download protocol |
IHC protocol for IFI16 antibody 67790-1-Ig | Download protocol |
IF protocol for IFI16 antibody 67790-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |