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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated IFI16 Monoclonal antibody

IFI16 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C10D7

Cat no : CL488-67790

Synonyms

Ifi 16, IFI16, IFNGIP1, PYHIN2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated IFI16 Monoclonal ant (CL488-67790)

    IF Staining of HeLa using CL488-67790

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 IFI16 antibody (CL488-67790, Clone: 1C10D7 ) at dilution of 1:400.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67790 targets IFI16 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IFI16 fusion protein Ag30314 相同性解析による交差性が予測される生物種
Full Name interferon, gamma-inducible protein 16
Calculated molecular weight 729 aa, 82 kDa
Observed molecular weight85-95 kDa
GenBank accession numberBC017059
Gene symbol IFI16
Gene ID (NCBI) 3428
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFI16 is a member of a family of interferon-inducible nuclear proteins involved in transcriptional regulation functions as a transcriptional repressor. IFI16 may be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. It has been reported that IFI16 participates in the regulation of autophagy, TP53-mediated cell death and innate immune response.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 IFI16 antibody CL488-67790Download protocol
Standard Protocols
Click here to view our Standard Protocols