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Species Reactivity

Species Reactivity

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Conjugate

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IFITM2-specific Monoclonal antibody

IFITM2-specific Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3D5F7

Cat no : CL488-66137

Synonyms

1 8D, IFITM2

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-66137

    FC experiment of HeLa using CL488-66137

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IFITM2-specific (CL488-66137, Clone:3D5F7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66137-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-66137 targets IFITM2-specific in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interferon induced transmembrane protein 2 (1-8D)
Observed molecular weight 15 kDa
GenBank accession numberNM_006435
Gene symbol IFITM2
Gene ID (NCBI) 10581
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death.(PMID:19544527) This antibody is specific to IFTIM2.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 IFITM2-specific antibody CL488-66137Download protocol
Standard Protocols
Click here to view our Standard Protocols