Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICC

WB analysis of HeLa using 17460-1-AP
WB result of IFT140 antibody (17460-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFT140 transfected HeLa cells.
WB analysis using 17460-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 17460-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of rat testis using 17460-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17460-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of rat testis using 17460-1-AP
IP result of anti-IFT140 (IP:17460-1-AP, 4ug; Detection:17460-1-AP 1:300) with rat testis tissue lysate 4400ug.
IHC staining of mouse testis using 17460-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17460-1-AP (IFT140 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skeletal muscle using 17460-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17460-1-AP (IFT140 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skeletal muscle using 17460-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17460-1-AP (IFT140 antibody) at dilution of 1:200 (under 40x lens).
IF Staining of mouse testis using 17460-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using IFT140 antibody (17460-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). DAPI (blue).
IF Staining of C2C12 using 17460-1-AP
Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using IFT140 antibody (17460-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

Featured Product
KD/KO Validated

IFT140 Polyclonal antibody

IFT140 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 17460-1-AP

Publications(81)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Synonyms

WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (81)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, mouse testis tissue, rat testis tissue
Positive IP detected in	rat testis tissue
Positive IHC detected in	mouse testis tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse testis tissue
Positive IF/ICC detected in	C2C12 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 26 publications below
IHC	See 4 publications below
IF	See 63 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

17460-1-AP targets IFT140 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, canine, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11119 Product name: Recombinant human IFT140 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1114-1462 aa of BC035577 Sequence: AEDLDETSDPALLARCSDFFIEHSQYERAVELLLAARKYQEALQLCLGQNMSITEEMAEKMTVAKDSSDLPEESRRELLEQIADCCMRQGSYHLATKKYTQAGNKLKAMRALLKSGDTEKITFFASVSRQKEIYIMAANYLQSLDWRKEPEIMKNIIGFYTKGRALDLLAGFYDACAQVEIDEYQNYDKAHGALTEAYKCLAKAKAKSPLDQETRLAQLQSRMALVKRFIQARRTYTEDPKESIKQCELLLEEPDLDSTIRIGDVYGFLVEHYVRKEEYQTAYRFLEEMRRRLPLANMSYYVSPQAVDAVHRGLGLPLPRTVPEQVRHNSMEDARELDEEVVEEADDDP 相同性解析による交差性が予測される生物種
Full Name	intraflagellar transport 140 homolog (Chlamydomonas)
Calculated molecular weight	1462 aa, 165 kDa
Observed molecular weight	150-165 kDa
GenBank accession number	BC035577
Gene Symbol	IFT140
Gene ID (NCBI)	9742
RRID	AB_2295648
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96RY7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)

Protocols

Product Specific Protocols
IF protocol for IFT140 antibody 17460-1-AP	Download protocol
IHC protocol for IFT140 antibody 17460-1-AP	Download protocol
IP protocol for IFT140 antibody 17460-1-AP	Download protocol
WB protocol for IFT140 antibody 17460-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Genet Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy. Authors - Carol-Anne Martin View Article
mouse	WB,IF	Cell Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision. Authors - Siew Cheng Phua View Article
		Hepatology Biallelic mutations in TTC26 (IFT56) cause severe biliary ciliopathy in humans. Authors - Ranad Shaheen View Article
human	IF	Sci Adv Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ. Authors - Chuan Chen View Article
human	IF	Nat Commun A distal centriolar protein network controls organelle maturation and asymmetry. Authors - Lei Wang View Article
mouse	WB	Nat Commun Kinesin-2 and IFT-A act as a complex promoting nuclear localization of β-catenin during Wnt signalling. Authors - Linh T Vuong View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 17460-1-AP

WB analysis using 17460-1-AP

WB analysis of mouse testis using 17460-1-AP

WB analysis of rat testis using 17460-1-AP

WB analysis of HepG2 using 17460-1-AP

IP experiment of rat testis using 17460-1-AP

IHC staining of mouse testis using 17460-1-AP

IHC staining of human skeletal muscle using 17460-1-AP

IHC staining of human skeletal muscle using 17460-1-AP

IF Staining of mouse testis using 17460-1-AP

IF Staining of C2C12 using 17460-1-AP

IFT140 Polyclonal antibody

IFT140 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.

Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.

Biallelic mutations in TTC26 (IFT56) cause severe biliary ciliopathy in humans.

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

A distal centriolar protein network controls organelle maturation and asymmetry.

Kinesin-2 and IFT-A act as a complex promoting nuclear localization of β-catenin during Wnt signalling.