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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated IFT140 Polyclonal antibody

IFT140 Polyclonal Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-17460

Synonyms

WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite®594-conjugated IFT140 Polyclonal antibody (CL594-17460)

    IF Staining of mouse testis using CL594-17460

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 IFT140 antibody (CL594-17460) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite®594-conjugated IFT140 Polyclonal antibody (CL594-17460)

    IF Staining of mouse testis using CL594-17460

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 IFT140 antibody (CL594-17460) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CoraLite®594-conjugated IFT140 Polyclonal antibody (CL594-17460)

    IF Staining of hTERT-RPE1 using CL594-17460

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite®594 IFT140 antibody (CL594-17460) at dilution of 1:200, CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

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Tested Applications

Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-17460 targets IFT140 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT140 fusion protein Ag11119 相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 140 homolog (Chlamydomonas)
Calculated molecular weight 1462 aa, 165 kDa
GenBank accession numberBC035577
Gene symbol IFT140
Gene ID (NCBI) 9742
RRIDAB_2919840
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)

Protocols

Product Specific Protocols
IF protocol for CL594 IFT140 antibody CL594-17460Download protocol
Standard Protocols
Click here to view our Standard Protocols