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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CoraLite® Plus 488-conjugated IFT140 Polyclonal an (CL488-17460)

    IF Staining of hTERT-RPE1 using CL488-17460

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite® Plus 488 IFT140 antibody (CL488-17460) at dilution of 1:200, ARL13B antibody (17711-1-AP, red).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IFT140 Polyclonal antibody

IFT140 Polyclonal Antibody for IF/ICC
Cat No. CL488-17460

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

c305C8.4, c380F5.1, DKFZp564L232, FLJ10306, FLJ30571, gs114, IFT140, KIAA0590, WDTC2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17460 targets IFT140 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT140 fusion protein Ag11119 相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 140 homolog (Chlamydomonas)
Calculated molecular weight 1462 aa, 165 kDa
GenBank accession numberBC035577
Gene Symbol IFT140
Gene ID (NCBI) 9742
RRIDAB_2919147
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RY7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 IFT140 antibody CL488-17460Download protocol
Standard Protocols
Click here to view our Standard Protocols