Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
Biotin-66408 targets Human IgG4 in IHC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | immunoglobulin heavy constant gamma 4 (G4m marker) |
| Calculated molecular weight | 55 kDa |
| GenBank accession number | AL928742 |
| Gene Symbol | IGHG4 |
| Gene ID (NCBI) | 3503 |
| RRID | AB_2883071 |
| Conjugate | Biotin |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4. This antibody is Biotin conjugated.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Biotin Human IgG4 antibody Biotin-66408 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |