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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Biotin-conjugated IgG4 Monoclonal antibody

Human IgG4 Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC

Conjugate

Biotin

CloneNo.

1A1A2

Cat no : Biotin-66408

Synonyms

IgG4, IGHG4

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Biotin-conjugated Human IgG4 Monoclonal antibody (Biotin-66408)

    IHC staining of human tonsillitis using Biotin-66408

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-66408 (IGHG4 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Biotin-conjugated Human IgG4 Monoclonal antibody (Biotin-66408)

    IHC staining of human tonsillitis using Biotin-66408

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-66408 (IGHG4 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-66408 targets Human IgG4 in IHC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant gamma 4 (G4m marker)
Calculated molecular weight 55 kDa
GenBank accession numberAL928742
Gene symbol IGHG4
Gene ID (NCBI) 3503
RRIDAB_2883071
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4. This antibody is Biotin conjugated.

Protocols

Product Specific Protocols
IHC protocol for Biotin Human IgG4 antibody Biotin-66408Download protocol
Standard Protocols
Click here to view our Standard Protocols