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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using HRP-conjugated Human IgG4 Monoclonal antibody (HRP-66408)

    WB analysis of human plasma using HRP-66408

    human plasma were subjected to SDS PAGE followed by western blot with HRP-66408 (human IgG4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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HRP-conjugated IgG4 Monoclonal antibody

Human IgG4 Monoclonal Antibody for WB
Cat No. HRP-66408
Clone No.1A1A2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Synonyms

IgG4, IGHG4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  HRP
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman plasma tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

HRP-66408 targets Human IgG4 in WB applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant gamma 4 (G4m marker)
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberAL928742
Gene Symbol IGHG4
Gene ID (NCBI) 3503
RRIDAB_2883839
Conjugate HRP
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4. This antibody is conjugated with HRP.

Protocols

Product Specific Protocols
WB protocol for HRP Human IgG4 antibody HRP-66408Download protocol
Standard Protocols
Click here to view our Standard Protocols