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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IL-18 Monoclonal antibody

IL-18 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, hamster

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5C6F8

Cat no : CL488-60070

Synonyms

IL18, Pre-IL18, IL-1 gamma, IL 18, IL 1 gamma

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated IL-18 Monoclonal ant (CL488-60070)

    IF Staining of HeLa using CL488-60070

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 IL-18 antibody (CL488-60070, Clone: 5C6F8 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated IL-18 Monoclonal ant (CL488-60070)

    FC experiment of HeLa using CL488-60070

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IL-18 (CL488-60070, Clone:5C6F8) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60070 targets IL-18 in IF/ICC, FC (Intra) applications and shows reactivity with human, hamster samples.

Tested Reactivity human, hamster
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IL-18 fusion protein Ag1063 相同性解析による交差性が予測される生物種
Full Name IL-18
Calculated molecular weight 193 aa, 22 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC007461
Gene symbol IL-18
Gene ID (NCBI) 3606
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 24 kDa precursor and then cleaved into a biologically activate 18 kDa form.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 IL-18 antibody CL488-60070Download protocol
FC protocol for CL Plus 488 IL-18 antibody CL488-60070Download protocol
Standard Protocols
Click here to view our Standard Protocols