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CoraLite®594-conjugated IL-18 Monoclonal antibody
IL-18 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
human, hamster
Applications
FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
5C6F8
Cat no : CL594-60070
Synonyms
Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | HeLa cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-60070 targets IL-18 in FC (Intra) applications and shows reactivity with human, hamster samples.
Tested Reactivity | human, hamster |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | IL-18 fusion protein Ag1063 相同性解析による交差性が予測される生物種 |
Full Name | IL-18 |
Calculated molecular weight | 193 aa, 22 kDa |
Observed molecular weight | 24 kDa |
GenBank accession number | BC007461 |
Gene symbol | IL-18 |
Gene ID (NCBI) | 3606 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 24 kDa precursor and then cleaved into a biologically activate 18 kDa form.
Protocols
Product Specific Protocols | |
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FC protocol for CL594 IL-18 antibody CL594-60070 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |