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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated JNK Monoclonal antibody

JNK Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A12E1

Cat no : CL488-66210

Synonyms

MAPK8, JNK1, JNK 2, JNK 1, EC:2.7.11.24

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CoraLite® Plus 488-conjugated JNK Monoclonal antib (CL488-66210)

    IF Staining of Jurkat using CL488-66210

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 JNK antibody (CL488-66210, Clone: 1A12E1 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated JNK Monoclonal antib (CL488-66210)

    FC experiment of HeLa using CL488-66210

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human JNK (CL488-66210, Clone:1A12E1) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66210 targets JNK in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen JNK fusion protein Ag21426 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 8
Calculated molecular weight 48 kDa
Observed molecular weight 44-48 kDa, 50-55 kDa
GenBank accession numberBC130572
Gene symbol JNK
Gene ID (NCBI) 5599
RRIDAB_2919300
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

  • JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.  

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 JNK antibody CL488-66210Download protocol
FC protocol for CL Plus 488 JNK antibody CL488-66210Download protocol
Standard Protocols
Click here to view our Standard Protocols