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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated JNK Monoclonal antibody

JNK Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A12E1

Cat no : CL594-66210

Synonyms

MAPK8, JNK1, JNK 2, JNK 1, EC:2.7.11.24

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CoraLite®594-conjugated JNK Monoclonal antibody (CL594-66210)

    IF Staining of Jurkat using CL594-66210

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite®594 JNK antibody (CL594-66210, Clone: 1A12E1 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated JNK Monoclonal antibody (CL594-66210)

    FC experiment of HeLa using CL594-66210

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human JNK (CL594-66210, Clone:1A12E1) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66210 targets JNK in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen JNK fusion protein Ag21426 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 8
Calculated molecular weight 48 kDa
Observed molecular weight 44-48 kDa, 50-55 kDa
GenBank accession numberBC130572
Gene symbol JNK
Gene ID (NCBI) 5599
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

  • JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.  

Protocols

Product Specific Protocols
IF protocol for CL594 JNK antibody CL594-66210Download protocol
FC protocol for CL594 JNK antibody CL594-66210Download protocol
Standard Protocols
Click here to view our Standard Protocols