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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite®488-conjugated LGALS3BP Monoclonal antibo (CL488-60066)

    IF Staining of A431 using CL488-60066

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®488 LGALS3BP antibody (CL488-60066, Clone: 2D8E11 ) at dilution of 1:200.

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CoraLite®488-conjugated LGALS3BP Monoclonal antibody

LGALS3BP Monoclonal Antibody for IF/ICC
Cat No. CL488-60066
Clone No.2D8E11

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Synonyms

90K, BTBD17B, Galectin 3 binding protein, LGALS3BP, M2BP, Mac 2 binding protein, MAC 2 BP, MAC2BP, Tumor associated antigen 90K

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60066 targets LGALS3BP in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen LGALS3BP fusion protein Ag0294 相同性解析による交差性が予測される生物種
Full Name lectin, galactoside-binding, soluble, 3 binding protein
Calculated molecular weight 585 aa, 65 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC002403
Gene Symbol LGALS3BP
Gene ID (NCBI) 3959
RRIDAB_2919228
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths491 nm / 516 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08380
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ). LGALS3BP has a predicted mass of ∼65 kDa. It is heavily glycosylated and shows an apparent molecular weight of 90 kDa on SDS-PAGE.

Protocols

Product Specific Protocols
IF protocol for CL488 LGALS3BP antibody CL488-60066Download protocol
Standard Protocols
Click here to view our Standard Protocols