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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated LGALS3BP Monoclonal antibody

LGALS3BP Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D8E11

Cat no : CL594-60066

Synonyms

90K, BTBD17B, Galectin 3 binding protein, LGALS3BP, M2BP, Mac 2 binding protein, MAC 2 BP, MAC2BP, Tumor associated antigen 90K

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite®594-conjugated LGALS3BP Monoclonal antibo (CL594-60066)

    IF Staining of human breast cancer using CL594-60066

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 LGALS3BP antibody (CL594-60066, Clone: 2D8E11 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite®594-conjugated LGALS3BP Monoclonal antibo (CL594-60066)

    IF Staining of human breast cancer using CL594-60066

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 LGALS3BP antibody (CL594-60066, Clone: 2D8E11 ) at dilution of 1:100.

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Tested Applications

Positive IF-P detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60066 targets LGALS3BP in IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen LGALS3BP fusion protein Ag0294 相同性解析による交差性が予測される生物種
Full Name lectin, galactoside-binding, soluble, 3 binding protein
Calculated molecular weight 585 aa, 65 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC002403
Gene symbol LGALS3BP
Gene ID (NCBI) 3959
RRIDAB_2919892
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ). LGALS3BP has a predicted mass of ∼65 kDa. It is heavily glycosylated and shows an apparent molecular weight of 90 kDa on SDS-PAGE.

Protocols

Product Specific Protocols
IF protocol for CL594 LGALS3BP antibody CL594-60066Download protocol
Standard Protocols
Click here to view our Standard Protocols