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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®532-conjugated Lamin B1 Monoclonal antibo (CL532-66095)

    IF Staining of HeLa using CL532-66095

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®532 Lamin B1 antibody (CL532-66095, Clone: 3C10G12 ) at dilution of 1:400.

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  • Featured Product
  • KD/KO Validated

CoraLite®532-conjugated Lamin B1 Monoclonal antibody

Lamin B1 Monoclonal Antibody for IF/ICC
Cat No. CL532-66095
Clone No.3C10G12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

LMNB1, LMNB, LMN2, Lamin-B1, laminB1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®532
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL532-66095 targets Lamin B1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Lamin B1 fusion protein Ag20522 相同性解析による交差性が予測される生物種
Full Name lamin B1
Calculated molecular weight 66 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC012295
Gene Symbol Lamin B1
Gene ID (NCBI) 4001
ENSEMBL Gene IDENSG00000113368
RRIDAB_2919582
Conjugate CoraLite®532 Fluorescent Dye
Excitation/Emission maxima wavelengths537 nm / 560 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20700
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.

Protocols

Product Specific Protocols
IF protocol for CL532 Lamin B1 antibody CL532-66095Download protocol
Standard Protocols
Click here to view our Standard Protocols