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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated Lamin B1 Monoclonal antibody

Lamin B1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3C10G12

Cat no : CL555-66095

Synonyms

LMNB1, LMNB, LMN2, Lamin-B1, laminB1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite®555-conjugated Lamin B1 Monoclonal antibo (CL555-66095)

    IF Staining of U2OS using CL555-66095

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite®555 Lamin B1 antibody (CL555-66095, Clone: 3C10G12 ) at dilution of 1:1000.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®555-conjugated Lamin B1 Monoclonal antibo (CL555-66095)

    IF Staining of HepG2 using CL555-66095

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 Lamin B1 antibody (CL555-66095, Clone: 3C10G12 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated Lamin B1 Monoclonal antibo (CL555-66095)

    FC experiment of HeLa using CL555-66095

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Lamin B1 (CL555-66095, Clone:3C10G12) (red), or 0.4 ug isotype control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inU2OS cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66095 targets Lamin B1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Lamin B1 fusion protein Ag20522 相同性解析による交差性が予測される生物種
Full Name lamin B1
Calculated molecular weight 66 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC012295
Gene symbol Lamin B1
Gene ID (NCBI) 4001
RRIDAB_2919674
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.

Protocols

Product Specific Protocols
IF protocol for CL555 Lamin B1 antibody CL555-66095Download protocol
FC protocol for CL555 Lamin B1 antibody CL555-66095Download protocol
Standard Protocols
Click here to view our Standard Protocols