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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Human M-CSF (3G12E6)

M-CSF Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3G12E6

Cat no : CL647-65283

Synonyms

M-CSF, PG-M-CSF, Processed macrophage colony-stimulating factor 1, Proteoglycan macrophage colony-stimulating factor, 3G12E6, CSF 1, CSF1, CSF-1, Lanimostim, M CSF, Macrophage colony-stimulating factor 1, Macrophage colony-stimulating factor 1 43 kDa subunit, MCSF

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human M-CSF (3G12E6) (CL647-65283)

    FC experiment of human PBMCs using CL647-65283

    1X10^6 LPS and TNF-α then Brefeldin A treated human PBMCs were surface stained with CoraLite® Plus 555 Anti-Human CD11b then intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human M-CSF (CL647-65283, Clone:3G12E6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human M-CSF (3G12E6) (CL647-65283)

    FC experiment of human PBMCs using CL647-65283

    1X10^6 LPS and TNF-α then Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human M-CSF (CL647-65283, Clone:3G12E6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated then Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human M-CSF (CL647-65283, Clone:3G12E6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

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Tested Applications

Positive FC (Intra) detected inLPS and Brefeldin A treated human PBMCs
Positive FC detected inLPS and Brefeldin A treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65283 targets M-CSF in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen M-CSF fusion protein HZ-1192 相同性解析による交差性が予測される生物種
Full Name colony stimulating factor 1 (macrophage)
Calculated molecular weight 60 kDa
GenBank accession numberBC021117
Gene symbol M-CSF
Gene ID (NCBI) 1435
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 M-CSF antibody CL647-65283Download protocol
Standard Protocols
Click here to view our Standard Protocols