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Perilipin 3/TIP47 Polyclonal antibody

Name: Perilipin 3/TIP47 Polyclonal antibody
Brand: Proteintech
SKU: 10694-1-AP
Rating: 4.00 (1 reviews)

Perilipin 3/TIP47 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 10694-1-AP

Synonyms

M6PRBP1, Perilipin 3, TIP47, Perilipin-3, Mannose-6-phosphate receptor-binding protein 1

Validation Data Gallery

Filter:

AllWBIPIF/ICCFC (Intra)

WB analysis using 10694-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10694-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10694-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10694-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10694-1-AP
IP result of anti-TIP47 (IP:10694-1-AP, 3ug; Detection:10694-1-AP 1:1000) with HeLa cells lysate 1000ug.
IF Staining of MEF cells using 10694-1-AP
IF result of anti-TIP47 (10694-1-AP,1:500) with MEF cell by Dr.Hector Alex Saka.
IF Staining of HeLa using 10694-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (10694-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
FC experiment of HeLa using 10694-1-AP
1X10^6 HeLa cells were stained with 0.2ug TIP47 antibody (10694-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Cat No. 10694-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, Jurkat cells, HeLa cells
Positive IP detected in	HeLa cells
Positive IF/ICC detected in	MEF cells, oleic acid treated HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 20 publications below
IHC	See 2 publications below
IF	See 8 publications below

Product Information

10694-1-AP targets Perilipin 3/TIP47 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, rat, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Perilipin 3/TIP47 fusion protein Ag1028 相同性解析による交差性が予測される生物種
Full Name	mannose-6-phosphate receptor binding protein 1
Calculated molecular weight	47 kDa
Observed molecular weight	47 kDa
GenBank accession number	BC007566
Gene symbol	TIP47/PLIN3
Gene ID (NCBI)	10226
RRID	AB_2297252
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.

Protocols

Product Specific Protocols
WB protocol for Perilipin 3/TIP47 antibody 10694-1-AP	Download protocol
IF protocol for Perilipin 3/TIP47 antibody 10694-1-AP	Download protocol
IP protocol for Perilipin 3/TIP47 antibody 10694-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Commun The autophagy protein ATG9A enables lipid mobilization from lipid droplets. Authors - Elodie Mailler View Article
human	WB	Hepatology CIDEC/Fsp27 is regulated by PPARα and plays a critical role in fasting- and diet-induced hepatosteatosis. Authors - Cédric Langhi View Article
human	IF	J Cell Biol A role for ubiquitin ligases and Spartin/SPG20 in lipid droplet turnover. Authors - Eastman Scott W SW View Article
	WB	J Hepatol Perilipin-5 is regulated by statins and controls triglyceride contents in the hepatocyte. Authors - Cédric Langhi View Article
human	WB	Cancer Res KRAS Controls Pancreatic Cancer Cell Lipid Metabolism and Invasive Potential through the Lipase HSL. Authors - Cody N Rozeveld View Article
human	WB	EMBO J COPI-TRAPPII activates Rab18 and regulates its lipid droplet association. Authors - Chunman Li View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Christine (Verified Customer) (09-19-2023)

Tested on HEK293T cells stimulated by 300 uM oleic acid overnight to stimulate the formation of lipid droplets. This antibody (tested only at 1:100 so far) decorates the lipid droplets, it worked well.

Applications: Immunofluorescence
Primary Antibody Dilution: 1:100
Cell Tissue Type: HEK293T

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Perilipin 3/TIP47 Polyclonal antibody

Perilipin 3/TIP47 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 10694-1-AP

WB analysis of Jurkat using 10694-1-AP

WB analysis of HEK-293 using 10694-1-AP

WB analysis of HeLa using 10694-1-AP

IP experiment of HeLa using 10694-1-AP

IF Staining of MEF cells using 10694-1-AP

IF Staining of HeLa using 10694-1-AP

FC experiment of HeLa using 10694-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The autophagy protein ATG9A enables lipid mobilization from lipid droplets.

CIDEC/Fsp27 is regulated by PPARα and plays a critical role in fasting- and diet-induced hepatosteatosis.

A role for ubiquitin ligases and Spartin/SPG20 in lipid droplet turnover.

Perilipin-5 is regulated by statins and controls triglyceride contents in the hepatocyte.

KRAS Controls Pancreatic Cancer Cell Lipid Metabolism and Invasive Potential through the Lipase HSL.

COPI-TRAPPII activates Rab18 and regulates its lipid droplet association.

Reviews

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