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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CoraLite®555-conjugated TIP47 Polyclonal antibody (CL555-10694)

    IF Staining of HUVEC using CL555-10694

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HUVEC cells using CoraLite®555 TIP47 antibody (CL555-10694) at dilution of 1:200.

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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated Perilipin 3/TIP47 Polyclonal antibody

Perilipin 3/TIP47 Polyclonal Antibody for IF/ICC
Cat No. CL555-10694

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

M6PRBP1, Perilipin 3, TIP47, Perilipin-3, Mannose-6-phosphate receptor-binding protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®555
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inoleic acid treated HUVEC cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-10694 targets Perilipin 3/TIP47 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Perilipin 3/TIP47 fusion protein Ag1028 相同性解析による交差性が予測される生物種
Full Name mannose-6-phosphate receptor binding protein 1
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC007566
Gene Symbol TIP47/PLIN3
Gene ID (NCBI) 10226
RRIDAB_2919591
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60664
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.

Protocols

Product Specific Protocols
IF protocol for CL555 Perilipin 3/TIP47 antibody CL555-10694Download protocol
Standard Protocols
Click here to view our Standard Protocols