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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TIP47 Polyclonal antibody

TIP47 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10694

Synonyms

M6PRBP1, Perilipin 3, TIP47, Perilipin-3, PLIN3, PP17, Mannose-6-phosphate receptor-binding protein 1, 47 kDa mannose 6-phosphate receptor-binding protein, 47 kDa MPR-binding protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CoraLite® Plus 488-conjugated TIP47 Polyclonal ant (CL488-10694)

    IF Staining of HUVEC using CL488-10694

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HUVEC cells using CoraLite® Plus 488 TIP47 antibody (CL488-10694) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated TIP47 Polyclonal ant (CL488-10694)

    IF Staining of HeLa using CL488-10694

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using CoraLite® Plus 488 TIP47 antibody (CL488-10694) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated TIP47 Polyclonal ant (CL488-10694)

    FC experiment of HeLa using CL488-10694

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TIP47 (CL488-10694) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inoleic acid treated HeLa cells, oleic acid treated HUVEC cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10694 targets TIP47 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIP47 fusion protein Ag1028 相同性解析による交差性が予測される生物種
Full Name mannose-6-phosphate receptor binding protein 1
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC007566
Gene symbol TIP47/PLIN3
Gene ID (NCBI) 10226
RRIDAB_2918992
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TIP47 antibody CL488-10694Download protocol
Standard Protocols
Click here to view our Standard Protocols