• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite®594-conjugated METTL3 Monoclonal antibody (CL594-67733)

    IF Staining of mouse testis using CL594-67733

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:400.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite®594-conjugated METTL3 Monoclonal antibody (CL594-67733)

    IF Staining of mouse testis using CL594-67733

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:400.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite®594-conjugated METTL3 Monoclonal antibody (CL594-67733)

    IF Staining of mouse testis using CL594-67733

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite®594-conjugated METTL3 Monoclonal antibody (CL594-67733)

    IF Staining of mouse testis using CL594-67733

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite®594-conjugated METTL3 Monoclonal antibody (CL594-67733)

    IF Staining of mouse testis using CL594-67733

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite®594-conjugated METTL3 Monoclonal antibody (CL594-67733)

    IF Staining of mouse testis using CL594-67733

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite®594-conjugated METTL3 Monoclonal antibody (CL594-67733)

    FC experiment of HEK-293 using CL594-67733

    1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human METTL3 (CL594-67733, Clone:2D8H1) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated METTL3 Monoclonal antibody

METTL3 Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL594-67733
Clone No.2D8H1
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Synonyms

MTA70, MT A70, Methyltransferase-like protein 3, hMETTL3, EC:2.1.1.348

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse testis tissue
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67733 targets METTL3 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen METTL3 fusion protein Ag7110 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 3
Calculated molecular weight 64 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC001650
Gene Symbol METTL3
Gene ID (NCBI) 56339
RRIDAB_2920176
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86U44
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

Protocols

Product Specific Protocols
IF protocol for CL594 METTL3 antibody CL594-67733Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC (Intra)

BMC Med

METTL3 facilitates kidney injury through promoting IRF4-mediated plasma cell infiltration via an m6A-dependent manner in systemic lupus erythematosus

Authors - Yu Liu
View Article