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  • Featured Product
  • KD/KO Validated

HRP-conjugated METTL3 Monoclonal antibody

METTL3 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB

Conjugate

HRP

CloneNo.

2D8H1

Cat no : HRP-67733

Synonyms

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HRP-conjugated METTL3 Monoclonal antibody (HRP-67733)

    WB analysis of HeLa using HRP-67733

    HeLa cells were subjected to SDS PAGE followed by western blot with HRP-67733 (METTL3 antibody) at dilutions of 1:5000, 1:10000 and 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HRP-conjugated METTL3 Monoclonal antibody (HRP-67733)

    WB analysis of HeLa using HRP-67733

    HeLa cells were subjected to SDS PAGE followed by western blot with HRP-67733 (METTL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

HRP-67733 targets METTL3 in WB applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen METTL3 fusion protein Ag7110 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 3
Calculated molecular weight 64 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC001650
Gene symbol METTL3
Gene ID (NCBI) 56339
RRIDAB_2920513
Conjugate HRP
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

Protocols

Product Specific Protocols
WB protocol for HRP METTL3 antibody HRP-67733Download protocol
Standard Protocols
Click here to view our Standard Protocols