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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated NAT10 Monoclonal antibody

NAT10 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3E3D8

Cat no : CL488-67465

Synonyms

N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 3E3D8

Validation Data Gallery

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  • Flow cytometry (FC) experiment of NIH/3T3 cells using CoraLite® Plus 488-conjugated NAT10 Monoclonal ant (CL488-67465)

    FC experiment of NIH/3T3 using CL488-67465

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Nat10 Monoclonal Antibody (CL488-67465, Clone:3E3D8)(red), or 0.8 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67465 targets NAT10 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NAT10 fusion protein Ag4355 相同性解析による交差性が予測される生物種
Full Name N-acetyltransferase 10 (GCN5-related)
Calculated molecular weight 1025 aa, 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC035558
Gene symbol NAT10
Gene ID (NCBI) 55226
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 NAT10 antibody CL488-67465Download protocol
Standard Protocols
Click here to view our Standard Protocols