Validation Data Gallery
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Tested Applications
| Positive FC (Intra) detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-67465 targets NAT10 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NAT10 fusion protein Ag4355 相同性解析による交差性が予測される生物種 |
| Full Name | N-acetyltransferase 10 (GCN5-related) |
| Calculated molecular weight | 1025 aa, 116 kDa |
| Observed molecular weight | 116 kDa |
| GenBank accession number | BC035558 |
| Gene Symbol | NAT10 |
| Gene ID (NCBI) | 55226 |
| RRID | AB_3674013 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 496 nm, 565 nm / 578 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H0A0 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE NAT10 antibody PE-67465 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |