ChromoTek Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, CoraLite® Plus 750 [CTK0105, CTK0106]
Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.
Cat no : smsG2bCL750-1
Synonyms
Validation Data Gallery
Product Information
Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.
| Description | Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below. |
| Applications | IF, WB |
| Host | Alpaca-derived, recombinantly produced in bacteria |
| Species Reactivity | Mouse IgG2b Fc-fragment No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG1, IgG2a, IgG2c, IgG3, IgM |
| Physical State | Liquid |
| Conjugate | CoraLite® Plus 750 |
| Suggested Dilution | Immunofluorescence 1: 500 - 1:1,000 Western blot 1: 500 - 1:1,000 |
| Purification Method | Recombinant expression, affinity purification IMAC |
| Type | Nanobody |
| Class | Recombinant |
| Affinity | CTK0105: KD = 5 nM, CTK0106: KD = 0.2 nM |
| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide |
| Storage Condition | Shipped at ambient temperature. Store at +4°C short term or -20°C long term. Stable for 1 year at -20°C. |
| RRID | AB_2941317 |
| Note | This product is for research use only, not for diagnostic or therapeutic use |
Documentation
| SDS |
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| SDS Alpaca anti-mouse IgG2b CoraLite® Plus 750 (PDF) |
| Datasheet |
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| Datasheet_Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, CoraLite® Plus 750 |
| Application note |
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| One-step Incubation with Nano-Secondaries (PDF) |
| Blocking recommendation for immunostaining with Nano-Secondaries (PDF) |
| Brochure |
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| Chromotek nanobodies brochure (PDF) |