Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.
Immunofluorescence 1:1,000 Western blot 1:1,000 Optimal working concentration is application-dependent and should be determined by testing a dilution range from 1:250 to 1:2,000. Note: Image acquisition time may have to be optimized.
Clonality:
Biclonal: mixture of 2 monoclonal Nanobodies
Host:
Alpaca
Affinity:
CTK0105: KD = 5 nM, CTK0106: KD = 0.2 nM
Specificity/Target:
Mouse IgG2b; this Nanobody mixture recognizes the Fc fragment of mouse IgG2b
Reactivity:
Mouse
Conjugate:
Alexa Fluor® 647
Storage Buffer:
10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
Storage Condition:
Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles.
