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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Occludin Recombinant antibody (80545-1-RR)

    WB analysis using 80545-1-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Occludin Recombinant antibody (80545-1-RR)

    WB analysis using 80545-1-RR

    HaCaT cells were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig colon tissue using Occludin Recombinant antibody (80545-1-RR)

    WB analysis of pig colon using 80545-1-RR

    pig colon tissue were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using Occludin Recombinant antibody (80545-1-RR)

    WB analysis of mouse colon using 80545-1-RR

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HUVEC cells using Occludin Recombinant antibody (80545-1-RR)

    IP experiment of HUVEC using 80545-1-RR

    IP result of anti-Occludin (IP:80545-1-RR, 4ug; Detection:80545-1-RR 1:15000) with HUVEC cells lysate 1410 ug.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Occludin Recombinant antibody (80545-1-RR)

    IF Staining of MCF-7 using 80545-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Occludin antibody (80545-1-RR, Clone: 8B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Affinity and Kinetic Characterization of 80545-1-RR

    Affinity and Kinetic Characterization of 80545-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80545-1-RR against Human Occludin were performed. The affinity constant is 26.5 pM.

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Occludin Recombinant antibody

Occludin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 80545-1-RR
Clone No.8B2

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Pig

Applications

WB, IF/ICC, IP, ELISA

Synonyms

OCLN, 8B2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHEK-293 cells, HaCaT cells, pig colon tissue, mouse colon tissue, HUVEC cells, U2OS cells
Positive IP detected inHUVEC cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80545-1-RR targets Occludin in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Pig samples.

Tested Reactivity Human, Mouse, Pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Occludin fusion protein Ag26173 相同性解析による交差性が予測される生物種
Full Name occludin
Calculated molecular weight 522 aa, 59 kDa
Observed molecular weight59 kDa
GenBank accession numberBC029886
Gene Symbol Occludin
Gene ID (NCBI) 4950
RRIDAB_2918901
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16625
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Protocols

Product Specific Protocols
WB protocol for Occludin antibody 80545-1-RRDownload protocol
IF protocol for Occludin antibody 80545-1-RRDownload protocol
IP protocol for Occludin antibody 80545-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols