CoraLite® Plus 488-conjugated Occludin Recombinant antibody
Occludin Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
8B2
Cat no : CL488-80545
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-80545 targets Occludin in IF/ICC applications and shows reactivity with Human, Mouse, Pig samples.
| Tested Reactivity | Human, Mouse, Pig |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Occludin fusion protein Ag26173 相同性解析による交差性が予測される生物種 |
| Full Name | occludin |
| Calculated molecular weight | 522 aa, 59 kDa |
| Observed molecular weight | 59 kDa |
| GenBank accession number | BC029886 |
| Gene symbol | Occludin |
| Gene ID (NCBI) | 4950 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Occludin antibody CL488-80545 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
