Validation Data Gallery

Filter: AllWBIPIHCIF-PIF-FroIF/ICC

WB analysis of SKOV-3 using 10336-1-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10336-1-AP (PAX8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10336-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10336-1-AP (PAX8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10336-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10336-1-AP (PAX8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10336-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10336-1-AP (PAX8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of SKOV-3 using 10336-1-AP
IP result of anti-PAX8 (IP:10336-1-AP, 4ug; Detection:10336-1-AP 1:15000) with SKOV-3 cells lysate 3280 ug.
IP experiment of HeLa cells using 10336-1-AP
IP result of anti-PAX8 (10336-1-AP for IP and Detection) with Hela cell.
IHC staining of human ovary cancer using 10336-1-AP
Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 10336-1-AP (PAX8 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 10336-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10336-1-AP (PAX8 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 10336-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10336-1-AP (PAX8 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 10336-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10336-1-AP (PAX8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 10336-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10336-1-AP (PAX8 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 10336-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10336-1-AP (PAX8 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 10336-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10336-1-AP (PAX8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse kidney using 10336-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using PAX8 antibody (10336-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse kidney using 10336-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using 10336-1-AP (PAX8 antibody) at dilution of 1:600 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of SKOV-3 using 10336-1-AP
Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PAX8 antibody (10336-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Featured Product
KD/KO Validated

PAX8 Polyclonal antibody

PAX8 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 10336-1-AP

Publications(526)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (5)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Synonyms

PAX-8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8, paired box 8

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (526)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	SKOV-3 cells, HEK-293 cells, Raji cells, NIH/3T3 cells
Positive IP detected in	SKOV-3 cells, HeLa cells
Positive IHC detected in	human ovary cancer tissue, human renal cell carcinoma tissue, human thyroid cancer tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse kidney tissue
Positive IF/ICC detected in	SKOV-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:800-1:3200
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 61 publications below
IHC	See 378 publications below
IF	See 110 publications below
ELISA	See 1 publications below
FC	See 1 publications below
CoIP	See 1 publications below

Product Information

10336-1-AP targets PAX8 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, rabbit, zebrafish, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	fusion protein 相同性解析による交差性が予測される生物種
Full Name	paired box 8
Calculated molecular weight	48 kDa
Observed molecular weight	48 kDa, 58 kDa
GenBank accession number	BC001060
Gene Symbol	PAX8
Gene ID (NCBI)	7849
RRID	AB_2236705
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q06710
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952). Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.

Protocols

Product Specific Protocols
WB protocol for PAX8 antibody 10336-1-AP	Download protocol
IHC protocol for PAX8 antibody 10336-1-AP	Download protocol
IF protocol for PAX8 antibody 10336-1-AP	Download protocol
IP protocol for PAX8 antibody 10336-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,CoIP	Nature The renal lineage factor PAX8 controls oncogenic signalling in kidney cancer. Authors - Saroor A Patel KD Validated View Article
human	IHC	Nat Biotechnol Tubuloids derived from human adult kidney and urine for personalized disease modeling. Authors - Frans Schutgens View Article
human	IHC	Nat Med Combined mutation in Vhl, Trp53 and Rb1 causes clear cell renal cell carcinoma in mice. Authors - Sabine Harlander View Article
human,mouse	WB,IHC	Cancer Discov A Targetable Myeloid Inflammatory State Governs Disease Recurrence in Clear Cell Renal Cell Carcinoma. Authors - Phillip M Rappold View Article
human	IHC	Cell Stem Cell Patient-derived organoids model cervical tissue dynamics and viral oncogenesis in cervical cancer. Authors - Kadi Lõhmussaar View Article
human	IF	Cell Stem Cell Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network. Authors - Jian Hui Low View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of SKOV-3 using 10336-1-AP

WB analysis of Raji using 10336-1-AP

WB analysis of NIH/3T3 using 10336-1-AP

WB analysis of HEK-293 using 10336-1-AP

IP experiment of SKOV-3 using 10336-1-AP

IP experiment of HeLa cells using 10336-1-AP

IHC staining of human ovary cancer using 10336-1-AP

IHC staining of human thyroid cancer using 10336-1-AP

IHC staining of human thyroid cancer using 10336-1-AP

IHC staining of mouse kidney using 10336-1-AP

IHC staining of human renal cell carcinoma using 10336-1-AP

IHC staining of human renal cell carcinoma using 10336-1-AP

IHC staining of mouse kidney using 10336-1-AP

IF Staining of mouse kidney using 10336-1-AP

IF Staining of mouse kidney using 10336-1-AP

IF Staining of SKOV-3 using 10336-1-AP

PAX8 Polyclonal antibody

PAX8 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The renal lineage factor PAX8 controls oncogenic signalling in kidney cancer.

Tubuloids derived from human adult kidney and urine for personalized disease modeling.

Combined mutation in Vhl, Trp53 and Rb1 causes clear cell renal cell carcinoma in mice.

A Targetable Myeloid Inflammatory State Governs Disease Recurrence in Clear Cell Renal Cell Carcinoma.

Patient-derived organoids model cervical tissue dynamics and viral oncogenesis in cervical cancer.

Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.