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PAX8 Polyclonal antibody
PAX8 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (5)
Applications
WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA
Conjugate
Unconjugated
518
Cat no : 10336-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | SKOV-3 cells, HEK-293 cells, Raji cells, NIH/3T3 cells |
| Positive IP detected in | SKOV-3 cells, HeLa cells |
| Positive IHC detected in | human ovary cancer tissue, human renal cell carcinoma tissue, human thyroid cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
| Positive IF/ICC detected in | SKOV-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 56 publications below |
| IHC | See 376 publications below |
| IF | See 109 publications below |
| ELISA | See 1 publications below |
| FC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
10336-1-AP targets PAX8 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, rabbit, zebrafish, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | fusion protein 相同性解析による交差性が予測される生物種 |
| Full Name | paired box 8 |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 48 kDa, 58 kDa |
| GenBank accession number | BC001060 |
| Gene symbol | PAX8 |
| Gene ID (NCBI) | 7849 |
| RRID | AB_2236705 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.
This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952). Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PAX8 antibody 10336-1-AP | Download protocol |
| IHC protocol for PAX8 antibody 10336-1-AP | Download protocol |
| IF protocol for PAX8 antibody 10336-1-AP | Download protocol |
| IP protocol for PAX8 antibody 10336-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature The renal lineage factor PAX8 controls oncogenic signalling in kidney cancer.
| ||
Nat Biotechnol Tubuloids derived from human adult kidney and urine for personalized disease modeling. | ||
Nat Med Combined mutation in Vhl, Trp53 and Rb1 causes clear cell renal cell carcinoma in mice. | ||
Cancer Discov A Targetable Myeloid Inflammatory State Governs Disease Recurrence in Clear Cell Renal Cell Carcinoma. | ||
Cell Stem Cell Patient-derived organoids model cervical tissue dynamics and viral oncogenesis in cervical cancer. | ||
Cell Stem Cell Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kenzo (Verified Customer) (03-28-2024) | Worked well for immunolabeling on mouse retina tissue sections.
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FH Eunyoung (Verified Customer) (02-28-2020) | This antibody works well on the mouse ovarian cancer tissue
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FH shuang (Verified Customer) (02-18-2020) | This Pax8 antibody works great for both IHC and IF staining on mouse Fallopian tube/uterus organoids and tumors, Highly recommend!
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FH Adam (Verified Customer) (05-30-2019) | Antibody works great for our applications (IHC on FFPE tissues). Only comment is that it cane sometimes have some background staining, particularly in some mouse tissues such as liver.
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FH Huai-Chin (Verified Customer) (04-15-2019) | So far only try the antibody for IHC on mouse tissue. Works pretty good. Would like to try it on human tissue/cell lines.
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FH Paulo (Verified Customer) (03-04-2019) | Used to perform ChIP-seq in ccRCC cell lines. 17.5ug of antibody used in 100million cells. IP was validated by WB. Sequencing detected PAX8 binding genome wide. Motif analysis identified the consensus PAX8 binding motif in approximately 90% of detected peaks.Would be great if Proteintech would produce a more concentrated version of this antibody for ChIP-seq.
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