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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated PAX8 Polyclonal antibody

PAX8 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10336

Synonyms

paired box 8, Paired box protein Pax 8, PAX8

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using CoraLite®594-conjugated PAX8 Polyclonal antibody (CL594-10336)

    IF Staining of SKOV-3 using CL594-10336

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite®594 PAX8 antibody (CL594-10336) at dilution of 1:200.

  • Flow cytometry (FC) experiment of SKOV-3 cells using CoraLite®594-conjugated PAX8 Polyclonal antibody (CL594-10336)

    FC experiment of SKOV-3 using CL594-10336

    1X10^6 SKOV-3 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human PAX8 (CL594-10336) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inSKOV-3 cells
Positive FC (Intra) detected inSKOV-3 cells
Positive FC detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10336 targets PAX8 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAX8 fusion protein Ag0306 相同性解析による交差性が予測される生物種
Full Name paired box 8
Calculated molecular weight 48 kDa
Observed molecular weight
GenBank accession numberBC001060
Gene symbol PAX8
Gene ID (NCBI) 7849
RRIDAB_2919728
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952). Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.

Protocols

Product Specific Protocols
IF protocol for CL594 PAX8 antibody CL594-10336Download protocol
Standard Protocols
Click here to view our Standard Protocols