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CoraLite® Plus 647-conjugated PAX8 Polyclonal antibody
PAX8 Polyclonal Antibody for IF/ICC, IF-P
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IF-P
Conjugate
CoraLite® Plus 647 Fluorescent Dye
Cat no : CL647-10336
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | mouse kidney tissue |
| Positive IF/ICC detected in | SKOV-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-10336 targets PAX8 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PAX8 fusion protein Ag0306 相同性解析による交差性が予測される生物種 |
| Full Name | paired box 8 |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 48 kDa, 58 kDa |
| GenBank accession number | BC001060 |
| Gene symbol | PAX8 |
| Gene ID (NCBI) | 7849 |
| RRID | AB_2920213 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.
This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952). Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 PAX8 antibody CL647-10336 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Janett (Verified Customer) (07-03-2024) | Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human fallopian tube tissue using CoraLite® Plus 647 PAX8 antibody (CL647-10336). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) in a steamer for 20min and Antibody Dilution 1:50
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