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  • Featured Product
  • KD/KO Validated

PCM1 Polyclonal antibody

PCM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 19856-1-AP

Synonyms

hPCM 1, PCM 1, PCM1, pericentriolar material 1, PTC4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCM1 Polyclonal antibody (19856-1-AP)

    WB analysis using 19856-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19856-1-AP (PCM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PCM1 Polyclonal antibody (19856-1-AP)

    WB analysis of NIH/3T3 using 19856-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19856-1-AP (PCM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using PCM1 Polyclonal antibody (19856-1-AP)

    WB analysis of HT-1080 using 19856-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 19856-1-AP (PCM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using PCM1 Polyclonal antibody (19856-1-AP)

    IP experiment of HeLa cells using 19856-1-AP

    IP result of anti-PCM1 (19856-1-AP for IP and Detection) with HeLa cells.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PCM1 Polyclonal antibody (19856-1-AP)

    IHC staining of human tonsillitis using 19856-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19856-1-AP (PCM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PCM1 Polyclonal antibody (19856-1-AP)

    IHC staining of human tonsillitis using 19856-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19856-1-AP (PCM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PCM1 Polyclonal antibody (19856-1-AP)

    IHC staining of mouse testis using 19856-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19856-1-AP (PCM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PCM1 Polyclonal antibody (19856-1-AP)

    IHC staining of mouse testis using 19856-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19856-1-AP (PCM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PCM1 Polyclonal antibody (19856-1-AP)

    IF Staining of HepG2 using 19856-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 19856-1-AP (PCM1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PCM1 Polyclonal antibody (19856-1-AP)

    IF Staining of HeLa using 19856-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PCM1 antibody (19856-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8, red).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using PCM1 Polyclonal antibody (19856-1-AP)

    IF Staining of NIH/3T3 using 19856-1-AP

    Immunofluorescent analysis of (-20℃Methanol) fixed NIH/3T3 cells using 19856-1-AP (PCM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, HT-1080 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, NIH/3T3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19856-1-AP targets PCM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCM1 fusion protein Ag13929 相同性解析による交差性が予測される生物種
Full Name pericentriolar material 1
Calculated molecular weight 2024 aa, 228 kDa
Observed molecular weight 260-280 kDa
GenBank accession numberBC000453
Gene symbol PCM1
Gene ID (NCBI) 5108
RRIDAB_2878616
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).

Protocols

Product Specific Protocols
WB protocol for PCM1 antibody 19856-1-APDownload protocol
IHC protocol for PCM1 antibody 19856-1-APDownload protocol
IF protocol for PCM1 antibody 19856-1-APDownload protocol
FC protocol for PCM1 antibody 19856-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Authors - Xin Li
View Article
mouseWB

Dev Cell

ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.

Authors - Jie Ran
View Article
humanWB

J Cell Biol

A ciliopathy complex builds distal appendages to initiate ciliogenesis.

Authors - Dhivya Kumar
  • KO Validated
View Article
human,mouseIF

Elife

Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis

Authors - Emma A Hall
View Article
humanWB

EMBO Rep

Aurora Kinase A proximity map reveals centriolar satellites as regulators of its ciliary function.

Authors - Melis D Arslanhan
View Article
mouseIHC

Cardiovasc Diabetol

Diastolic dysfunction in a pre-clinical model of diabetes is associated with changes in the cardiac non-myocyte cellular composition.

Authors - Charles D Cohen
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Julie (Verified Customer) (01-06-2020)

This antibody works great and reliably with PFA fix & methanol permeabilization.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: primary fibroblasts