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CoraLite®555-conjugated PCM1 Polyclonal antibody
PCM1 Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF
Conjugate
CoraLite®555 Fluorescent Dye
Cat no : CL555-19856
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
The immunogen of CL555-19856 is PCM1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PCM1 fusion protein Ag13929 相同性解析による交差性が予測される生物種 |
| Full Name | pericentriolar material 1 |
| Calculated molecular weight | 2024 aa, 228 kDa |
| Observed molecular weight | 260-280 kDa |
| GenBank accession number | BC000453 |
| Gene symbol | PCM1 |
| Gene ID (NCBI) | 5108 |
| RRID | AB_2919636 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 557 nm / 570nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 PCM1 antibody CL555-19856 | Download protocol |
| FC protocol for CL555 PCM1 antibody CL555-19856 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
