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CoraLite® Plus 488-conjugated PDH E1 Alpha Monoclonal antibody
PDH E1 Alpha Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
WB
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2B3C10
Cat no : CL488-66119
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, HEK-293 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of CL488-66119 is PDH E1 Alpha Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | PDH E1 Alpha fusion protein Ag12556 相同性解析による交差性が予測される生物種 |
Full Name | pyruvate dehydrogenase (lipoamide) alpha 1 |
Calculated molecular weight | 43 kDa |
Observed molecular weight | 43 kDa |
GenBank accession number | BC002406 |
Gene symbol | PDHA1 |
Gene ID (NCBI) | 5160 |
RRID | AB_2919287 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).
Protocols
Product Specific Protocols | |
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WB protocol for CL Plus 488 PDH E1 Alpha antibody CL488-66119 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |