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CoraLite®594-conjugated PDH E1 Alpha Monoclonal antibody
PDH E1 Alpha Monoclonal Antibody for WB, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
WB, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2B3C10
Cat no : CL594-66119
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-66119 targets PDH E1 Alpha in WB, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PDH E1 Alpha fusion protein Ag12556 相同性解析による交差性が予測される生物種 |
| Full Name | pyruvate dehydrogenase (lipoamide) alpha 1 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 43 kDa |
| GenBank accession number | BC002406 |
| Gene symbol | PDHA1 |
| Gene ID (NCBI) | 5160 |
| RRID | AB_2919949 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL594 PDH E1 Alpha antibody CL594-66119 | Download protocol |
| FC protocol for CL594 PDH E1 Alpha antibody CL594-66119 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
