CoraLite® Plus 647-conjugated PKC Gamma Polyclonal antibody
PKC Gamma Polyclonal Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB
Conjugate
CoraLite® Plus 647 Fluorescent Dye
Cat no : CL647-14364
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-14364 targets PKC Gamma in WB applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PKC Gamma fusion protein Ag5681 相同性解析による交差性が予測される生物種 |
| Full Name | protein kinase C, gamma |
| Calculated molecular weight | 78 kDa |
| Observed molecular weight | 76-80 kDa |
| GenBank accession number | BC047876 |
| Gene symbol | PKC Gamma |
| Gene ID (NCBI) | 5582 |
| RRID | AB_2920225 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 647 PKC Gamma antibody CL647-14364 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
