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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLCL2 Monoclonal antibody

PLCL2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C8A4

Cat no : 67471-1-Ig

Synonyms

Phospholipase C-L2, Phospholipase C-epsilon-2, Phospholipase C L2, KIAA1092, Inactive phospholipase C-like protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLCL2 Monoclonal antibody (67471-1-Ig)

    WB analysis using 67471-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67471-1-Ig (PLCL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PLCL2 Monoclonal antibody (67471-1-Ig)

    IF Staining of HeLa using 67471-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67471-1-Ig (PLCL2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inpig brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67471-1-Ig targets PLCL2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLCL2 fusion protein Ag11461 相同性解析による交差性が予測される生物種
Full Name phospholipase C-like 2
Calculated molecular weight 126 kDa
Observed molecular weight 126 kDa
GenBank accession numberBC036392
Gene symbol PLCL2
Gene ID (NCBI) 23228
RRIDAB_2882700
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLCL2, a novel phospholipase C-like protein, is expressed in lymphocytes and platelets. The expression of PLCL2 is associated with the proliferation of mature B cells in the immune system. PLCL2 is a new susceptibility loci for myocardial infarction. It has been shown that PLCL2 plays a key role in the pathogenesis of atherosclerosis and systemic sclerosisit. There are three isoforms of PLCL2 protein and 67471-1-Ig antibody detects the 126 kDa band in SDS-PAGE. (PMID: 24916648, 25880423)

Protocols

Product Specific Protocols
WB protocol for PLCL2 antibody 67471-1-IgDownload protocol
IF protocol for PLCL2 antibody 67471-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols