Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67471 targets PLCL2 in IF/ICC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PLCL2 fusion protein Ag11461 相同性解析による交差性が予測される生物種 |
| Full Name | phospholipase C-like 2 |
| Calculated molecular weight | 126 kDa |
| Observed molecular weight | 126 kDa |
| GenBank accession number | BC036392 |
| Gene Symbol | PLCL2 |
| Gene ID (NCBI) | 23228 |
| RRID | AB_2919481 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UPR0 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLCL2, a novel phospholipase C-like protein, is expressed in lymphocytes and platelets. The expression of PLCL2 is associated with the proliferation of mature B cells in the immune system. PLCL2 is a new susceptibility loci for myocardial infarction. It has been shown that PLCL2 plays a key role in the pathogenesis of atherosclerosis and systemic sclerosisit. (PMID: 24916648, 25880423)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PLCL2 antibody CL488-67471 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |