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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated PLCL2 Monoclonal antibody

PLCL2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse, Pig

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C8A4

Cat no : CL594-67471

Synonyms

FLJ13484, KIAA1092, Phospholipase C epsilon 2, Phospholipase C L2, phospholipase C like 2, PLC epsilon 2, PLC L(2), PLC L2, PLCE2, PLCL2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated PLCL2 Monoclonal antibody (CL594-67471)

    IF Staining of HepG2 using CL594-67471

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 PLCL2 antibody (CL594-67471, Clone: 1C8A4 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67471 targets PLCL2 in IF/ICC applications and shows reactivity with Human, Rat, Mouse, Pig samples.

Tested Reactivity Human, Rat, Mouse, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLCL2 fusion protein Ag11461 相同性解析による交差性が予測される生物種
Full Name phospholipase C-like 2
Calculated molecular weight 126 kDa
Observed molecular weight 126 kDa
GenBank accession numberBC036392
Gene symbol PLCL2
Gene ID (NCBI) 23228
RRIDAB_2920142
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLCL2, a novel phospholipase C-like protein, is expressed in lymphocytes and platelets. The expression of PLCL2 is associated with the proliferation of mature B cells in the immune system. PLCL2 is a new susceptibility loci for myocardial infarction. It has been shown that PLCL2 plays a key role in the pathogenesis of atherosclerosis and systemic sclerosisit. There are three isoforms of PLCL2 protein and 67471-1-Ig antibody detects the 126 kDa band in SDS-PAGE. (PMID: 24916648, 25880423)

Protocols

Product Specific Protocols
IF protocol for CL594 PLCL2 antibody CL594-67471Download protocol
Standard Protocols
Click here to view our Standard Protocols