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Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using PLEK Monoclonal antibody (66431-1-Ig)

    WB analysis of U-937 using 66431-1-Ig

    WB result of PLEK antibody (66431-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLEK transfected U-937 cells.

  • Western Blot (WB) analysis of HL-60 cells using PLEK Monoclonal antibody (66431-1-Ig)

    WB analysis of HL-60 using 66431-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using PLEK Monoclonal antibody (66431-1-Ig)

    WB analysis of THP-1 using 66431-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using PLEK Monoclonal antibody (66431-1-Ig)

    WB analysis of U-937 using 66431-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig spleen tissue using PLEK Monoclonal antibody (66431-1-Ig)

    WB analysis of pig spleen using 66431-1-Ig

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PLEK Monoclonal antibody (66431-1-Ig)

    IHC staining of human colon cancer using 66431-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66431-1-Ig (PLEK Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PLEK Monoclonal antibody (66431-1-Ig)

    IHC staining of human colon cancer using 66431-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66431-1-Ig (PLEK Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using PLEK Monoclonal antibody (66431-1-Ig)

    IF Staining of mouse spleen using 66431-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using PLEK antibody (66431-1-Ig, Clone: 1D5C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using PLEK Monoclonal antibody (66431-1-Ig)

    IF Staining of mouse spleen using 66431-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using PLEK antibody (66431-1-Ig, Clone: 1D5C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  • Featured Product
  • KD/KO Validated

PLEK Monoclonal antibody

PLEK Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66431-1-Ig
Clone No.1D5C2
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

P47, Platelet 47 kDa protein, pleckstrin, PLEK

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, pig spleen tissue, THP-1 cells, U-937 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66431-1-Ig targets PLEK in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig, mouse samples.

Tested Reactivity human, pig, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLEK fusion protein Ag3187 相同性解析による交差性が予測される生物種
Full Name pleckstrin
Calculated molecular weight 350 aa, 40 kDa
Observed molecular weight 40-47 kDa
GenBank accession numberBC018549
Gene Symbol PLEK
Gene ID (NCBI) 5341
RRIDAB_2881802
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08567
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)

Protocols

Product Specific Protocols
WB protocol for PLEK antibody 66431-1-IgDownload protocol
IHC protocol for PLEK antibody 66431-1-IgDownload protocol
IF protocol for PLEK antibody 66431-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Aging (Albany NY)

Comprehensive analysis identifies crucial genes associated with immune cells mediating progression of carotid atherosclerotic plaque

Authors - Zhen Li
View Article