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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated PLEK Monoclonal antibody

PLEK Monoclonal Antibody for WB, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D5C2

Cat no : CL594-66431

Synonyms

pleckstrin, P47, 1D5C2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite®594-conjugated PLEK Monoclonal antibody (CL594-66431)

    WB analysis using CL594-66431

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-66431 (PLEK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated PLEK Monoclonal antibody (CL594-66431)

    FC experiment of HeLa using CL594-66431

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PLEK (CL594-66431, Clone:1D5C2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inU-937 cells, THP-1 cells, HL-60 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66431 targets PLEK in WB, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLEK fusion protein Ag3187 相同性解析による交差性が予測される生物種
Full Name pleckstrin
Calculated molecular weight 350 aa, 40 kDa
Observed molecular weight 40-47 kDa
GenBank accession numberBC018549
Gene symbol PLEK
Gene ID (NCBI) 5341
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)

Protocols

Product Specific Protocols
WB protocol for CL594 PLEK antibody CL594-66431Download protocol
FC protocol for CL594 PLEK antibody CL594-66431Download protocol
Standard Protocols
Click here to view our Standard Protocols