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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated PLEK Monoclonal anti (CL488-66431)

    WB analysis using CL488-66431

    HL-60 cells were subjected to SDS PAGE followed by western blot with CL488-66431 (PLEK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

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CoraLite® Plus 488-conjugated PLEK Monoclonal antibody

PLEK Monoclonal Antibody for WB
Cat No. CL488-66431
Clone No.1D5C2

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB

Synonyms

FLJ27168, P47, Platelet 47 kDa protein, pleckstrin, PLEK

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, THP-1 cells, U-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66431 targets PLEK in WB applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLEK fusion protein Ag3187 相同性解析による交差性が予測される生物種
Full Name pleckstrin
Calculated molecular weight 350 aa, 40 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC018549
Gene Symbol PLEK
Gene ID (NCBI) 5341
RRIDAB_3084245
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08567
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 PLEK antibody CL488-66431Download protocol
Standard Protocols
Click here to view our Standard Protocols